Histochemical and immunohistochemical techniques Field of study: Biology
Programme code: W2-S2BI19.2020

Module name: Histochemical and immunohistochemical techniques
Module code: 2BL_65a
Programme code: W2-S2BI19.2020
Semester:
  • winter semester 2021/2022
  • winter semester 2020/2021
Language of instruction: depending on the choice
Form of verification: course work
ECTS credits: 4
Description:
Detailed classification of histo- and immunohistological methods used in laboratories; introduction to terminology, characteristic of methods and the theoretical principles of methods including principles of fixation, dehydratation, embedding and sectioning for plant and animal tissues; skills in carrying out histo- and immunohistological reactions, recognition of analysed structures; improvement in interpretation of obtained results; student get the advanced knowledge in fluorescence and transmission electron microscopy techniques.
Prerequisites:
Knowledge of plant and animal histology, cell biology.
Key reading:
like in syllabus
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
Good knowledge concerning histochemical techniques used in plant and animal tissue analysis. [2BL_65_1]
 2BL_W01_P [4/5] 2BL_W07_P [4/5] 2BL_U01_P [4/5] 2BL_U04_P [4/5]
Knowledge of the advanced techniques in tissue analysis. [2BL_65_2]
 2BL_W04_P [4/5] 2BL_U01_P [4/5]
Classification and collection of data during carrying the histo- and immunohistochemical reactions. [2BL_65_3]
 2BL_U01_P [4/5] 2BL_U03_P [3/5]
Use the advanced techniques in plant and animal tissue analysis. [2BL_65_4]
 2BL_U01_P [4/5] 2BL_U03_P [4/5]
Abilities to carry out histo- and immunohistochemical staining. [2BL_65_5]
 2BL_U04_P [3/5] 2BL_U06_P [3/5]
Handling experimental observation and data interpretation of obtained data and reactions. [2BL_65_6]
 2BL_U02_P [3/5] 2BL_U06_P [3/5]
Present reasoned explanation of phenomena and problems, concerned tissue analysis. [2BL_65_7]
 2BL_W02_P [4/5] 2BL_U06_P [4/5] 2BL_K01_P [4/5]
Skills in practical use of presented histochemical techniques. [2BL_65_8]
 2BL_W04_P [4/5] 2BL_U03_P [4/5]
Type Description Codes of the learning outcomes of the module to which assessment is related
credit for a grade [2BL_65_w1]
Credit for the grade based on the rules specified in the syllabus.
 2BL_65_1 2BL_65_2 2BL_65_3 2BL_65_4 2BL_65_5 2BL_65_6 2BL_65_7 2BL_65_8
Form of teaching Student's own work Assessment of the learning outcomes
Type Description (including teaching methods) Number of hours Description Number of hours
lecture [2BL_65_fs_1]
Lecture chosen problems with the use of audiovisual methods; computer presentation illustrating the issues discussed.
 10
Work with the literature indicated by the teacher.
 10 credit for a grade [2BL_65_w1]
laboratory classes [2BL_65_fs_2]
Work under supervision of teacher – carrying out histochemical and immunohistochemical reactions to analyse plant and animal tissues following carefully a sequence of instruction provides by teacher; microscopic observation of specimens produced during classes (notes, drawings), discussion. Laboratory with the use of bright field and fluorescence microscopy, and transmission electron microscopy.
 50
Knowledge with understanding of the topics solved during laboratory; writing report, work with the literature indicated by the teacher.
 30 credit for a grade [2BL_65_w1]
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)