In vitro cultures in biotechnology Field of study: Biotechnology
Programme code: 01-S1BT12.2015

Module name: In vitro cultures in biotechnology
Module code: 1BT_17
Programme code: 01-S1BT12.2015
Semester: summer semester 2015/2016
Language of instruction: Polish
Form of verification: course work
ECTS credits: 5
Description:
The module delivers the advanced knowledge on cell and tissues cultures of plants and animals; acquaints the student with the principles of working in sterile conditions and the requirements and specifics of the laboratory for in vitro culture of plant and animal tissues. Particular emphasis is placed on mastering the various methods of in vitro cells/tissues culture of plants and knowledge of their practical use, including plant breeding. In addition, the biology of animal cell cultures is presented and the possibilities of their use in medical biotechnology are discussed. In experiments carried out independently, the student acquires the ability to work under aseptic conditions to establish, maintain, monitor and analyze plant cultures; collect empirical data and improves the ability to analyze and interpret the results of the observation.
Prerequisites:
Knowledge of botany, zoology and physiology on high school level.
Key reading:
see syllabus compulsory reading
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
Student is able to plan a basic laboratory equipment to conduct in vitro cultures of plant and animal cells [1BT_17_1]
 1BT_W07 [5/5] 1BT_W17 [5/5]
Student classifies and describes techniques for plant and animal cell/ tissues cultures, and defines their application in various fields of biotechnology and basic research. [1BT_17_2]
 1BT_W11 [5/5] 1BT_W14 [5/5] 1BT_W17 [5/5]
Student distinguishes between the types of in vitro cultures, classifies fundamental processes occurring in vitro in culture of plant cells/tissues and defines the conditions leading to a specific type of plant morphogenesis. [1BT_17_3]
 1BT_W05 [5/5] 1BT_W17 [5/5] 1BT_W18 [5/5]
Students applies the basic techniques of in vitro cultures and defines the conditions for the growth and differentiation of cells under in vitro culture conditions in different plant species. [1BT_17_4]
 1BT_U01 [5/5] 1BT_U04 [5/5] 1BT_U07 [5/5]
Student is able to evaluate different plant cultures in terms of their degree of differentiation, regeneration capacity and somaclonal variations. [1BT_17_5]
 1BT_U04 [5/5] 1BT_U06 [5/5]
Student describes the effects of the experiment, analyzes the results, draws conclusions and present them in a report. [1BT_17_6]
 1BT_U04 [5/5]
The student has a habit of knowledge updating and critical evaluation of the possibility of its practical use. [1BT_17_7]
 1BT_U04 [2/5] 1BT_K04 [2/5]
Student follows the rules of work in a specialized laboratory and takes care of their own and others safety. [1BT_17_8]
 1BT_K03 [5/5]
Type Description Codes of the learning outcomes of the module to which assessment is related
Final test [1BT_17_w_1]
The written work of verifying the level of understanding, knowledge and skills acquired in the laboratories and the issues discussed during the lectures.
 1BT_17_1 1BT_17_2 1BT_17_3 1BT_17_4 1BT_17_5
Evaluation of practical skills [1BT_17_w_2]
Verification of compliance with the rules of the work in specialized laboratories, including the maintenance of aseptic working conditions, the practical skills, an observation of the experiment and draw conclusions.
 1BT_17_6 1BT_17_7 1BT_17_8
Presentation of the report of the laboratory work [1BT_17_w_3]
Prepare a report describing the experiment, the obtained results and the presentation of the conclusions.
 1BT_17_5 1BT_17_6
Form of teaching Student's own work Assessment of the learning outcomes
Type Description (including teaching methods) Number of hours Description Number of hours
lecture [1BT_17_fs_1]
Lectures supported with computer presentations in Power Point to illustrate the problems discussed.
 15
Acquisition of knowledge presented in lectures; textbooks and research papers related to the topics discussed
 35
laboratory classes [1BT_17_fs_2]
Working under the supervision of the lecturer - performing experiments based on the instructions, the analysis of the results. Consultations: Individual work with the student on the preparation of the report of laboratory work.
45
Preparation for laboratory tasks based on the instructions and recommended literature.
 30 Final test [1BT_17_w_1] Evaluation of practical skills [1BT_17_w_2] Presentation of the report of the laboratory work [1BT_17_w_3]
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)