Histochemical and immunohistochemical techniques Field of study: Biotechnology
Programme code: 01-S2BTA14.2019

Module name: Histochemical and immunohistochemical techniques
Module code: 2BT_E_24
Programme code: 01-S2BTA14.2019
Semester:
  • summer semester 2021/2022
  • winter semester 2021/2022
  • summer semester 2020/2021
  • winter semester 2020/2021
  • summer semester 2019/2020
Language of instruction: English
Form of verification: course work
ECTS credits: 6
Description:
Detailed classification of histo- and immunohistological methods used in laboratories; introduction to terminology, characteristic of methods and the theoretical principles of methods including principles of fixation, dehydratation, embeddind and sectioning for plant and animal tissues; skils in carrying out histo- and immunohistological reactions, recognition of analysed structures; improvement in interpretation of obtained results; student get the advanced knowledge in fluorescence and transmission electron microscopy techniques.
Prerequisites:
Knowledge of plant and animal histology, cell biology.
Key reading:
see: syllabus compulsory reading
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
Good knowledge concerning histochemical techniques used in plant and animal tissues analysis. [2BT_E_24_01]
 2BT_E_W01_P [4/5] 2BT_E_W02_P [4/5] 2BT_E_W04_P [4/5] 2BT_E_W09_P [4/5] 2BT_E_U01_P [4/5]
Knowledge of the advanced techniques in tissues analysis. [2BT_E_24_02]
 2BT_E_W02_P [5/5] 2BT_E_W04_P [5/5] 2BT_E_W09_P [5/5]
Classification and collection of data during carrying the histo- and immunihistochemical reactions. [2BT_E_24_03]
 2BT_E_U02_P [4/5] 2BT_E_U03_P [4/5]
Use the advanced techniques in plant and animal tissues analysis. [2BT_E_24_04]
 2BT_E_W04_P [4/5] 2BT_E_U01_P [4/5]
Abilities to carry out histo- and immunohistochemical staining. [2BT_E_24_05]
 2BT_E_U01_P [3/5] 2BT_E_U03_P [3/5]
Handling experimental observation and data Interpretation of obtained data and reactions. [2BT_E_24_06]
 2BT_E_U03_P [3/5] 2BT_E_U06_P [3/5] 2BT_E_K02_P [3/5]
Present reasoned explanation of phenomena and problems, concerned tissues analysis. [2BT_E_24_07]
 2BT_E_U06_P [4/5] 2BT_E_K02_P [4/5]
Skils in practical use of presented histochemical techniques. [2BT_E_24_08]
 2BT_E_U06_P [3/5]
Type Description Codes of the learning outcomes of the module to which assessment is related
Written report [2BT_E_24_w01]
Written report by each of student after each of laboratory: description of histochemical and /or immunohistochemical techniques used for tissues analysis, expected effects, detection capabilities of analyzed structures, photo-documentation of reactions.
 2BT_E_24_01 2BT_E_24_02 2BT_E_24_03 2BT_E_24_06 2BT_E_24_07
Test of practical skills [2BT_E_24_w02]
Student's experimental skills will be evaluated on each laboratory by lecturel – know how to use apparatus, techniques according to protocols, principles of microscope, analysis and identification tissue structure, the quality of speciemens, interpretation and evaluation of obtainet results, evaluation of the ability to discuss problems raised during the laboratory work.
 2BT_E_24_03 2BT_E_24_04 2BT_E_24_05 2BT_E_24_06 2BT_E_24_08
Written pass test [2BT_E_24_w03]
Final completion of laboratory – completion of each laboratory and theory from lectures.
 2BT_E_24_01 2BT_E_24_02 2BT_E_24_04 2BT_E_24_08
Form of teaching Student's own work Assessment of the learning outcomes
Type Description (including teaching methods) Number of hours Description Number of hours
lecture [2BT_E_24_fs01]
Lectrure choosen problems with the use of audiovisual methods; computer presentation ilustrating the discussed issues.
 10
Work with manuals and suplementarny material.
 40 Written pass test [2BT_E_24_w03]
laboratory classes [2BT_E_24_fs02]
Work under supervision of teacher – carrying out histochemical and immunohistochemical reactions to analyse plant and animal tissues following carefully a sequence of instruction provides by teacher; microscopical observation of specimens produced during classes (notes, drawings) discussion. Laboratory with the use of bright field and fluorescence microscopy, and transmission electron microscopy.
 50
Knowledge with understanding of the topics solved during laboratory; writing report, work with the manuals.
 50 Written report [2BT_E_24_w01] Test of practical skills [2BT_E_24_w02]
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)