Patch-clamp technique in the study of plant cells Field of study: Biotechnology
Programme code: 01-S2BTA14.2017

Module name: Patch-clamp technique in the study of plant cells
Module code: 2BT_E_57
Programme code: 01-S2BTA14.2017
Semester:
  • summer semester 2018/2019
  • winter semester 2018/2019
  • summer semester 2017/2018
  • winter semester 2017/2018
Language of instruction: English
Form of verification: course work
ECTS credits: 2
Description:
The module of patch-clamp technique in the study of plant cells enables to gain the specialized knowledge, theoretical and practical scope of the patch-clamp method in the cells and tissues of plants. By this module the student will be acquainted with the physical fundamentals of transport through biological membranes, types of membrane ion transport systems (pumps, ion channels, transporters), electrophysiological techniques that serve to record currents flowing through biological membranes, analysis of currents flowing through a single ion channel and a system of signal transduction in plant cells with the participation of ion channels. In addition, the student acquires the skills to carry out measurements using the patch-clamp technique.
Prerequisites:
Knowledge of botany and plant physiology at the undergraduate level.
Key reading:
see: syllabus compulsory reading
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
Defines and describes the basic processes responsible for the transport of ions through biological membranes. [2BT_E_57_1]
 2BT_E_W02 [5/5] 2BT_E_U03 [4/5] 2BT_E_U09 [3/5]
Describes the types of membrane ion transport systems (pumps, ion channels, transporters). [2BT_E_57_2]
 2BT_E_W02 [5/5] 2BT_E_U03 [4/5] 2BT_E_U09 [4/5] 2BT_E_K01 [5/5]
Identifies the basic configurations of measuring patch-clamp techniques and analyzes the data obtained during measurements. [2BT_E_57_3]
 2BT_E_W14 [5/5] 2BT_E_U04 [5/5] 2BT_E_U05 [4/5] 2BT_E_K03 [4/5]
Conducts measurements by patch-clamp method in selected configurations and interprets the results. [2BT_E_57_4]
 2BT_E_W14 [5/5] 2BT_E_U04 [5/5] 2BT_E_K03 [5/5]
Type Description Codes of the learning outcomes of the module to which assessment is related
Ocena aktywności [2BT_E_57_w_1]
During practicals the following skills will be assessed: the use of laboratory equipment, proper experiment implementation, interpreting the results and conclusions drawing, preparing a written final report of the experiments performed.
 2BT_E_57_3 2BT_E_57_4
Kolokwium zaliczeniowe [2BT_E_57_w_2]
It covers issues discussed in lectures and information contained in recommended literature.
 2BT_E_57_1 2BT_E_57_2 2BT_E_57_3
Form of teaching Student's own work Assessment of the learning outcomes
Type Description (including teaching methods) Number of hours Description Number of hours
lecture [2BT_E_57_fs_1]
Lectures using multimedia devices.
 5
Knowledge of the material from the lectures and work with the literature positions recommended by the tutors.
 15 Kolokwium zaliczeniowe [2BT_E_57_w_2]
laboratory classes [2BT_E_57_fs_2]
Working in the patch-clamp laboratory, the measurements using different patch-clamp configuration, discussion and analysis of obtained results.
 20
Preparing for courses basing on the recommended literature. Preparing the final reports.
 10 Ocena aktywności [2BT_E_57_w_1]
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)