Fundamentals of in vitro cultures Field of study: Biology
Programme code: W2-S1BI19.2025

Module name: Fundamentals of in vitro cultures
Module code: 1BL_23_81
Programme code: W2-S1BI19.2025
Semester: winter semester 2026/2027
Language of instruction: depending on the choice
Form of verification: course work
ECTS credits: 3
Purpose and description of the content of education:
The module provides knowledge of the aseptic cultures of plant cells and tissues. The requirements of cells and tissues in in vitro culture are presented, and the factors determining the growth and morphogenesis of cells/tissues are discussed. The module familiarizes with various methods of plant regeneration and the possibilities of their use in practice and basic research. In self-conducted experiments, the student acquires the ability to work in aseptic conditions, masters the technique of establishing, maintaining, and analyzing cell/tissue cultures, collects empirical data, and improves the ability to analyze and interpret the results obtained based on the conducted observations
List of modules that must be completed before starting this module (if necessary): not applicable
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
Classifies types of in vitro cultures of plant cells and tissues and defines their usefulness for various biotechnological purposes and in basic research. [1BL_23_81_1]
 1BL_W02 [4/5] 1BL_W03 [4/5]
Distinguishes the basic morphogenetic processes occurring in the in vitro culture of plant cells/tissues and defines the culture conditions leading to a specific type of morphogenesis. [1BL_23_81_2]
 1BL_W03 [4/5] 1BL_W04 [4/5]
Applies basic techniques of in vitro cultures of plants and determines the conditions for cell growth and differentiation in in vitro cultures of various plant species. [1BL_23_81_3]
 1BL_W01 [5/5] 1BL_W04 [5/5] 1BL_W05 [5/5] 1BL_W06 [5/5]
Describes the experiment's effects, analyze the results, draw conclusions, and present them as a report. [1BL_23_81_4]
 1BL_U09 [5/5]
Updates knowledge in the field of in vitro culture techniques and their applications. [1BL_23_81_5]
 1BL_K01 [5/5]
Form of teaching Number of hours Methods of conducting classes Assessment of the learning outcomes Learning outcomes
lecture [1BL_23_81_w_1] 10 Formal lecture/ course-related lecture [a01] 
Problem-based lecture [b01] 
Activating method – discussion / debate [b04] 
Screen presentation [c07] 
Self-education [f01] 
Individual work with a text [f02]  		course work 1BL_23_81_1 1BL_23_81_2 1BL_23_81_3 1BL_23_81_5
laboratory classes [1BL_23_81_w_2] 20 Laboratory exercise / experiment [e01] 
Individual work with a text [f02]  		course work 1BL_23_81_1 1BL_23_81_2 1BL_23_81_3 1BL_23_81_4
The student's work, apart from participation in classes, includes in particular:
Name Category Description
Search for materials and review activities necessary for class participation [a01] Preparation for classes
reviewing literature, documentation, tools and materials as well as the specifics of the syllabus and the range of activities indicated in it as required for full participation in classes
Literature reading / analysis of source materials [a02] Preparation for classes
reading the literature indicated in the syllabus; reviewing, organizing, analyzing and selecting source materials to be used in class
Consulting materials complementary to those indicated in the syllabus [a04] Preparation for classes
agreeing on materials complementary to those indicated in the syllabus, supporting the implementation of tasks resulting from or necessary for class participation
Getting acquainted with the syllabus content [b01] Consulting the curriculum and the organization of classes
reading through the syllabus and getting acquainted with its content
Verification / adjustment / discussion of syllabus provisions [b02] Consulting the curriculum and the organization of classes
consulting the content of the syllabus, possibly in the presence of the year tutor or members of the class group, and, if necessary, reassessing the provisions concerning special conditions for class participation, e.g., space and time requirements, technical and other requirements, including conditions for participation in classes outside the walls of the university, classes organized in blocks, organized online, etc.
Determining the stages of task implementation contributing to the verification of learning outcomes [c01] Preparation for verification of learning outcomes
devising a task implementation strategy embracing the division of content, the range of activities, implementation time and/or the method(s) of obtaining the necessary materials and tools, etc.
Studying the literature used in and the materials produced in class [c02] Preparation for verification of learning outcomes
exploring the studied content, inquiring, considering, assimilating, interpreting it, or organizing knowledge obtained from the literature, documentation, instructions, scenarios, etc., used in class as well as from the notes or other materials/artifacts made in class
Implementation of an individual or group assignment necessary for course/phase/examination completion [c03] Preparation for verification of learning outcomes
a set of activities aimed at performing an assigned task, to be executed out of class, as an obligatory phase/element of the verification of the learning outcomes assigned to the course
Analysis of the corrective feedback provided by the academic teacher on the results of the verification of learning outcomes [d01] Consulting the results of the verification of learning outcomes
reading through the academic teacher’s comments, assessments and opinions on the implementation of the task aimed at checking the level of the achieved learning outcomes
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)