Spectrophotometry with elements of good laboratory practice Field of study: Biotechnology
Programme code: W2-S1BT19.2023

Module name: Spectrophotometry with elements of good laboratory practice
Module code: 1BT_23_59
Programme code: W2-S1BT19.2023
Semester:
  • winter semester 2024/2025
  • winter semester 2023/2024
Language of instruction: depending on the choice
Form of verification: course work
ECTS credits: 3
Purpose and description of the content of education:
The aim of the module is to familiarise the Student with good laboratory practice, enabling the proper design and execution of life science laboratory experiments. As part of the module, the student is introduced to the operation of basic laboratory apparatus and equipment. He/she learns the basic rules of working in the laboratory. He/she works in the laboratory maintaining safety rules. In the second part of the module, the student focuses on learning and understanding the foundations of spectroscopic methods for the range of near ultraviolet and visible light (UV-VIS), applicable in biotechnology. The student learns the theoretical foundations of these methods, the apparatus and its calibration methods, and masters sample preparation methods. The student also learns about the techniques of quantitative and qualitative spectrophotometric analysis, their sensitivity, accuracy, sources, and nature of errors in such measurements as well as ways to eliminate them. The student will become familiar with the possibilities and limitations of using UV-VIS spectrophotometry in biotechnology.
List of modules that must be completed before starting this module (if necessary): not applicable
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
Has knowledge of the fundamentals of chemical and physical UV-VIS spectroscopic methods. [1BT_23_59_01]
 1BT_W01 [4/5]
Lists and describes spectrophotometric methods and techniques used in biotechnology. [1BT_23_59_02]
 1BT_W01 [4/5]
Uses spectrophotometric methods and techniques in the quantitative and qualitative analysis of biological compounds [1BT_23_59_03]
 1BT_W02 [3/5] 1BT_W06 [4/5]
Demonstrates the ability to prepare samples, conduct analyses, process the results of conducted experiments, using statistical methods and evaluating them critically [1BT_23_59_04]
 1BT_W06 [3/5] 1BT_U03 [4/5]
Is able to work independently and in a team, knows and observes health and safety rules and the rules of work in the laboratory [1BT_23_59_05]
 1BT_U09 [4/5] 1BT_K01 [3/5]
Form of teaching Number of hours Methods of conducting classes Assessment of the learning outcomes Learning outcomes
laboratory classes [1 BT_23_59_L] 45 Description [a03] 
Demonstration-imitation [c06] 
Screen presentation [c07] 
Working with a computer [d01] 
Laboratory exercise / experiment [e01]  		course work 1BT_23_59_01 1BT_23_59_02 1BT_23_59_03 1BT_23_59_04 1BT_23_59_05
The student's work, apart from participation in classes, includes in particular:
Name Category Description
Search for materials and review activities necessary for class participation [a01] Preparation for classes
reviewing literature, documentation, tools and materials as well as the specifics of the syllabus and the range of activities indicated in it as required for full participation in classes
Literature reading / analysis of source materials [a02] Preparation for classes
reading the literature indicated in the syllabus; reviewing, organizing, analyzing and selecting source materials to be used in class
Consulting materials complementary to those indicated in the syllabus [a04] Preparation for classes
agreeing on materials complementary to those indicated in the syllabus, supporting the implementation of tasks resulting from or necessary for class participation
Production/preparation of tools, materials or documentation necessary for class participation [a05] Preparation for classes
developing, preparing and assessing the usefulness of tools and materials (e.g. aids, scenarios, research tools, equipment, etc.) to be employed in class or as an aid when preparing for classes
Getting acquainted with the syllabus content [b01] Consulting the curriculum and the organization of classes
reading through the syllabus and getting acquainted with its content
Determining the stages of task implementation contributing to the verification of learning outcomes [c01] Preparation for verification of learning outcomes
devising a task implementation strategy embracing the division of content, the range of activities, implementation time and/or the method(s) of obtaining the necessary materials and tools, etc.
Studying the literature used in and the materials produced in class [c02] Preparation for verification of learning outcomes
exploring the studied content, inquiring, considering, assimilating, interpreting it, or organizing knowledge obtained from the literature, documentation, instructions, scenarios, etc., used in class as well as from the notes or other materials/artifacts made in class
Implementation of an individual or group assignment necessary for course/phase/examination completion [c03] Preparation for verification of learning outcomes
a set of activities aimed at performing an assigned task, to be executed out of class, as an obligatory phase/element of the verification of the learning outcomes assigned to the course
Analysis of the corrective feedback provided by the academic teacher on the results of the verification of learning outcomes [d01] Consulting the results of the verification of learning outcomes
reading through the academic teacher’s comments, assessments and opinions on the implementation of the task aimed at checking the level of the achieved learning outcomes
Development of a corrective action plan as well as supplementary/corrective tasks [d02] Consulting the results of the verification of learning outcomes
reviewing and selecting tasks and activities enabling the elimination of errors indicated by the academic teacher, their verification or correction resulting in completing the task with at least the minimum passing grade
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)