In vitro cultures in biotechnology Field of study: Biotechnology
Programme code: W2-S1BT19.2023

Module name: In vitro cultures in biotechnology
Module code: 1BT_23_26
Programme code: W2-S1BT19.2023
Semester:
  • summer semester 2024/2025
  • summer semester 2023/2024
Language of instruction: depending on the choice
Form of verification: course work
ECTS credits: 4
Purpose and description of the content of education:
The module provides specialist knowledge in cell and tissue cultures of plants and animals; acquaints the student with the principles of working in sterile conditions as well as the requirements and specificity of the laboratory for in vitro cultures of plant and animal tissues. Particular emphasis is placed on mastering various methods of in vitro cultures of plant cells/tissues and learning about the possibilities of their practical use, including in plant breeding. In addition, the biology of animal cell cultures is presented, and the options for their use in medical biotechnology are discussed. In self-conducted experiments, the student acquires the skills to work in aseptic conditions, masters the technique of establishing, maintaining, monitoring, and analyzing plant cultures, collects empirical data and improves the ability to analyze and interpret the results of the conducted observations.
List of modules that must be completed before starting this module (if necessary): not applicable
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
The student classifies and describes the techniques of plant and animal cell and tissue cultures and defines their application in various fields of biotechnology and basic research. [1BT_23_26_1]
 1BT_W03 [5/5] 1BT_W06 [5/5]
Distinguishes types of in vitro cultures, characterize the basic properties of cells/tissues in vitro, classifies the basic morphogenetic processes occurring in the in vitro culture of plants, and define the culture conditions leading to a specific type of morphogenesis. [1BT_23_26_2]
 1BT_W03 [5/5] 1BT_W06 [5/5]
Applies basic techniques of in vitro cultures, determines the conditions enabling the growth and differentiation of cells in vitro in various plant species, and evaluates cultures of plant cells and tissues regarding the degree of differentiation, regenerative capacity, and somaclonal changes. [1BT_23_26_3]
 1BT_U04 [5/5] 1BT_U05 [5/5] 1BT_U09 [5/5]
Describes the experiment's effects, analyze the results, conclude, and present them as a report. [1BT_23_26_4]
 1BT_U04 [5/5]
The student observes work rules in a specialized laboratory and cares for the safety of his and others' work. [1BT_23_26_5]
 1BT_U09 [5/5] 1BT_K01 [3/5]
Form of teaching Number of hours Methods of conducting classes Assessment of the learning outcomes Learning outcomes
lecture [1BT_23_26_w_1] 15 Formal lecture/ course-related lecture [a01] 
Activating method – discussion / debate [b04] 
Screen presentation [c07] 
Self-education [f01] 
Individual work with a text [f02]  		course work 1BT_23_26_1 1BT_23_26_2 1BT_23_26_3
laboratory classes [1BT_23_26_w_2] 45 Screen presentation [c07] 
Laboratory exercise / experiment [e01] 
Individual work with a text [f02]  		course work 1BT_23_26_1 1BT_23_26_2 1BT_23_26_3 1BT_23_26_4 1BT_23_26_5
The student's work, apart from participation in classes, includes in particular:
Name Category Description
Search for materials and review activities necessary for class participation [a01] Preparation for classes
reviewing literature, documentation, tools and materials as well as the specifics of the syllabus and the range of activities indicated in it as required for full participation in classes
Literature reading / analysis of source materials [a02] Preparation for classes
reading the literature indicated in the syllabus; reviewing, organizing, analyzing and selecting source materials to be used in class
Consulting materials complementary to those indicated in the syllabus [a04] Preparation for classes
agreeing on materials complementary to those indicated in the syllabus, supporting the implementation of tasks resulting from or necessary for class participation
Getting acquainted with the syllabus content [b01] Consulting the curriculum and the organization of classes
reading through the syllabus and getting acquainted with its content
Verification / adjustment / discussion of syllabus provisions [b02] Consulting the curriculum and the organization of classes
consulting the content of the syllabus, possibly in the presence of the year tutor or members of the class group, and, if necessary, reassessing the provisions concerning special conditions for class participation, e.g., space and time requirements, technical and other requirements, including conditions for participation in classes outside the walls of the university, classes organized in blocks, organized online, etc.
Determining the stages of task implementation contributing to the verification of learning outcomes [c01] Preparation for verification of learning outcomes
devising a task implementation strategy embracing the division of content, the range of activities, implementation time and/or the method(s) of obtaining the necessary materials and tools, etc.
Studying the literature used in and the materials produced in class [c02] Preparation for verification of learning outcomes
exploring the studied content, inquiring, considering, assimilating, interpreting it, or organizing knowledge obtained from the literature, documentation, instructions, scenarios, etc., used in class as well as from the notes or other materials/artifacts made in class
Implementation of an individual or group assignment necessary for course/phase/examination completion [c03] Preparation for verification of learning outcomes
a set of activities aimed at performing an assigned task, to be executed out of class, as an obligatory phase/element of the verification of the learning outcomes assigned to the course
Analysis of the corrective feedback provided by the academic teacher on the results of the verification of learning outcomes [d01] Consulting the results of the verification of learning outcomes
reading through the academic teacher’s comments, assessments and opinions on the implementation of the task aimed at checking the level of the achieved learning outcomes
Development of a corrective action plan as well as supplementary/corrective tasks [d02] Consulting the results of the verification of learning outcomes
reviewing and selecting tasks and activities enabling the elimination of errors indicated by the academic teacher, their verification or correction resulting in completing the task with at least the minimum passing grade
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)