Histochemical and immunohistochemical techniques Field of study: Biotechnology
Programme code: W2-S2BT19.2022

Module name: Histochemical and immunohistochemical techniques
Module code: 2BT_24A
Programme code: W2-S2BT19.2022
Semester:
  • winter semester 2025/2026
  • winter semester 2024/2025
  • winter semester 2023/2024
  • winter semester 2022/2023
Language of instruction: depending on the choice
Form of verification: course work
ECTS credits: 4
Description:
Detailed classification of histo- and immunohistological methods used in laboratories; introduction to terminology, characteristic of methods and the theoretical principles of methods including principles of fixation, dehydratation, embedding and sectioning for plant and animal tissues; skills in carrying out histo- and immunohistological reactions, recognition of analysed structures; improvement in interpretation of obtained results; student get the advanced knowledge in fluorescence and transmission electron microscopy techniques.
Prerequisites:
Knowledge of plant and animal histology, cell biology
Key reading:
according to the Syllabus
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
Good knowledge concerning histochemical techniques used in plant and animal tissue analysis [2BT_24_01]
 2BT_W01_P [4/5] 2BT_W02_P [4/5] 2BT_W04_P [4/5] 2BT_W09_P [4/5] 2BT_U01_P [4/5]
Knowledge of the advanced techniques in tissue analysis [2BT_24_02]
 2BT_W02_P [5/5] 2BT_W04_P [4/5] 2BT_W09_P [4/5]
Classification and collection of data during carrying the histo- and immunohistochemical reactions [2BT_24_03]
 2BT_U02_P [4/5] 2BT_U03_P [4/5]
Use the advanced techniques in plant and animal tissue analysis [2BT_24_04]
 2BT_W04_P [4/5] 2BT_U01_P [3/5]
Abilities to carry out histo- and immunohistochemical staining [2BT_24_05]
 2BT_U01_P [3/5] 2BT_U03_P [3/5]
Handling experimental observation and data interpretation of obtained data and reactions [2BT_24_06]
 2BT_U03_P [3/5] 2BT_U06_P [3/5] 2BT_K02_P [3/5]
Present reasoned explanation of phenomena and problems, concerned tissue analysis [2BT_24_07]
 2BT_U06_P [4/5] 2BT_K02_P [4/5]
Skills in practical use of presented histochemical techniques [2BT_24_08]
 2BT_U06_P [3/5]
Type Description Codes of the learning outcomes of the module to which assessment is related
Coursework [2BT_24_w_1]
according to the Syllabus
 2BT_24_01 2BT_24_02 2BT_24_03 2BT_24_04 2BT_24_05 2BT_24_06 2BT_24_07 2BT_24_08
Form of teaching Student's own work Assessment of the learning outcomes
Type Description (including teaching methods) Number of hours Description Number of hours
lecture [2BT_24_fs01]
Lecture chosen problems with the use of audiovisual methods; computer presentation illustrating the issues discussed
 10
Work with manuals and supplementary material
 10 Coursework [2BT_24_w_1]
laboratory classes [2BT_24_fs02]
Work under supervision of teacher – carrying out histochemical and immunohistochemical reactions to analyse plant and animal tissues following carefully a sequence of instruction provides by teacher; microscopic observation of specimens produced during classes (notes, drawings), discussion Laboratory with the use of bright field and fluorescence microscopy, and transmission electron microscopy
 50
Knowledge with understanding of the topics solved during laboratory; writing report, work with the manuals
 30 Coursework [2BT_24_w_1]
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)