Analysis of plant channels current by patch clamp technique Field of study: Biotechnology
Programme code: W2-S2BTA19.2021

Module name: Analysis of plant channels current by patch clamp technique
Module code: 2BT_E_57
Programme code: W2-S2BTA19.2021
Semester:
  • summer semester 2025/2026
  • winter semester 2025/2026
  • summer semester 2024/2025
  • winter semester 2024/2025
  • summer semester 2023/2024
  • winter semester 2023/2024
  • summer semester 2022/2023
  • winter semester 2022/2023
  • summer semester 2021/2022
  • winter semester 2021/2022
Language of instruction: English
Form of verification: course work
ECTS credits: 2
Description:
The module enables to gain the specialized knowledge and skills, of the patch-clamp method in measurements of ion current through plant cells membranes. By this module the student will be acquainted with the physical fundamentals of transport through biological membranes, types of plant membrane ion transport systems (pumps, ion channels, transporters), patch-clamp configurations that serve to record currents flowing through biological membranes, analysis of currents flowing through ion channels. In addition, the student acquires the skills to carry out measurements using the patch-clamp technique.
Prerequisites:
Knowledge of botany and plant physiology at the undergraduate level.
Key reading:
see: syllabus compulsory reading
Learning outcome of the module Codes of the learning outcomes of the programme to which the learning outcome of the module is related [level of competence: scale 1-5]
Defines and describes the processes responsible for the transport of ions through plant biological membranes [2BT_E_57_1]
 2BT_E_W03_P [5/5] 2BT_E_U02_P [5/5] 2BT_E_U05_P [5/5]
Describes and distinguishes the types of plant membrane ion transport systems (pumps, ion channels, transporters). [2BT_E_57_2]
 2BT_E_W01_P [5/5] 2BT_E_W02_P [5/5] 2BT_E_U02_P [5/5]
Identifies and describes configurations of measuring patch-clamp techniques and analyzes the data obtained during measurements. [2BT_E_57_3]
 2BT_E_W01_P [5/5] 2BT_E_W04_P [5/5] 2BT_E_U01_P [5/5] 2BT_E_U03_P [5/5] 2BT_E_U06_P [5/5]
Conducts measurements by patch-clamp method in selected configurations and interprets the results. [2BT_E_57_4]
 2BT_E_W01_P [4/5] 2BT_E_U02_P [4/5] 2BT_E_U03_P [4/5] 2BT_E_U04_P [5/5] 2BT_E_K01_P [4/5] 2BT_E_K02_P [4/5]
Type Description Codes of the learning outcomes of the module to which assessment is related
Continuous assessment of practical skills [2BT_E_57_w_1]
During practicals the following skills will be assessed: the use of laboratory equipment, proper experiment implementation, interpreting the results and conclusions drawing, preparing a written final report of the experiments performed.
 2BT_E_57_3 2BT_E_57_4
Final test [2BT_E_57_w_2]
It covers issues discussed in lectures and information contained in recommended literature.
 2BT_E_57_1 2BT_E_57_2 2BT_E_57_3
Form of teaching Student's own work Assessment of the learning outcomes
Type Description (including teaching methods) Number of hours Description Number of hours
lecture [2BT_E_57_fs_1]
Lectures using multimedia devices.
 5
Knowledge of the material from the lectures and work with the literature positions recommended by the tutors.
 15 Final test [2BT_E_57_w_2]
laboratory classes [2BT_E_57_fs_2]
Working in the patch-clamp laboratory, the measurements using different patch-clamp configuration, discussion and analysis of obtained results.
 20
Preparing for courses basing on the recommended literature. Preparing the final reports.
 10 Continuous assessment of practical skills [2BT_E_57_w_1]
Attachments
Module description (PDF)
Information concerning module syllabuses might be changed during studies.
Syllabuses (USOSweb)
Semester Module Language of instruction
(no information given)